Wounds and Biofilm Infections
The wound infection microenvironment
In wound infections, an intricate communicative network exists between host cells, extracellular matrix, microbes, and immune cells, which are regulated by complex chemical and mechanical cues.
Microbial biofilms are the single-most important cause of non-healing wounds
An in vitro platform that mimics the wound infection milieu
We have developed an in vitro, biomimetic fluid that aims to recapitulate key components and their functions present in the wound infection milieu. This platform lends itself well to study important wound infection parameters such as bacterial growth, biofilm formation and metabolic activity, cell migration and wound closure, and can importantly be leveraged to explore three-dimensional biofilm architecture in human relevant wound conditions.
Developing a human-relevant, bioengineered platform for wound bed studies and translational applications
Using a bioengineering 'lab-on-chip' approach, we have developed an in vitro platform that mimics the architecture and biophysical factors of the wound state. Developing using in house design, software, and mechanical components, this platform is designed to be suitable for microscopy and cell and microbial culture. It will provide insights into the dynamic wound infection microenvironment and can be leveraged to evaluate therapeutics for wound infections.
Funded by the Ramalingaswami Re-entry Fellowship, Department of Biotechnology, Govt. of India
Human-Relevant Pre-clinical Testing of Infection Therapeutics
A microengineered platform for accelerated and high-throughput testing of infection therapeutics, including anti-biofilm approaches
Developing a biomimetic wound infection platform for accelerated and high-throughput pre-clinical testing of infection therapeutics
We aim to develop an in vitro model of the wound infection state that lends itself well for high-throughput, accelerated, precise, reproducible, accurate and low-cost testing and development of infection therapeutics. This will not only serve as an alternative to animal testing, but also open a new paradigm in infection therapeutics development with the 'Test more, Fail fast' approach.
Funded by Innovative Young Biotechnologist Award (IYBA), Department of Biotechnology, Govt. of India
Bridging the 'Gap': Co-development of a human-relevant 3D wound model of multispecies biofilms for preclinical testing of therapeutics
With Dr. Joey Shepherd and team at the University of Sheffield, UK, we will bridge research expertise across our groups in UK and India, to co-develop a human-relevant 3D wound model of multispecies biofilms, as a potential alternative to animal-based pre-clinical testing.
Through this collaboration, in 12 months, we will have developed for the scientific community a laboratory platform that recapitulates the wound infection state with multispecies biofilm growth, in the presence of relevant host components.
Biofilms in chronic, non-transmissible infection states
Exploring the role of biofilms in tobacco-associated oral cancer
Tobacco-associated precancerous lesions exist and develop in a dynamic, oral microenvironment, characterized by distinct microbial signatures. These precancerous lesions are intricately associated with diverse, multi-species microbial biofilms, which influence key processes chronic inflammation and carcinogenesis. This offers the possibility that the oral microbiome (or biofilms) can be leveraged as a tool or target in the management of tobacco-associated precancerous lesions, thereby opening precision-based oral cancer therapeutics.
Effects of smokeless tobacco on oral multispecies bacterial-fungal biofilms
With Dr. Sudhanshu Patwardhan and team at the Centre for Health Research and Education (CHRE), UK, we are using in vitro studies to explore the effects of smokeless tobacco forms on the oral multispecies biofilm microenvironment.
Towards this, we are working very closely with dental surgeon Dr. Deepti Bandaru, Consultant, CHRE, UK.
Historical remedies for biofilms
'Ancientbiotics' for biofilms: Can we look back to move forward?
The stalled antibiotic pipeline, rise in antibiotic resistance, and recalcitrant state of biofilm infections prompts the urgent need for novel therapeutic approaches. However, biofilms have probably existed since the advent of microbial life, and so have infection therapeutics for common biofilm states such as wound, ocular, ear and dental infections. We identify, reconstitute and explore ancient remedies from historical Indian medicine, and evaluate them for anti-biofilm effects using contemporary scientific approaches.
Antibiofilm potential of historical African remedies for wounds
With Dr. Philip Builders at National Institute of Pharmaceutical Development and Research (NIPRD), Abuja, Nigeria, we will be working to analyze the anti-biofilm potential of traditional African remedies for wounds using the in vitro wound milieu developed by our group.
This project is funded by the Africa-India Mobility Fund (AIMF) and we are looking forward to the collaboration!
Potential other projects
Dissecting host-microbial dynamics in chronic wound infections, with special reference to immune cell dynamics
Using an in vitro, biomimetic model of the chronic wound-bed capillary interface, our research will study immune cell signaling and expression, under selective and precise conditions of the chronic wound infection state. This will provide invaluable insights into immune cell functioning and potential therapeutics towards the chronic wound infection state.
Long-term characterization of microbial populations from patients with chronic wound infection states
We are starting a research project that focuses on long-term characterization of microbial populations from patients with chronic wound infections. We are looking to collaborate with clinicians/clinical microbiologists/infectious disease specialists/surgeons and academic faculty.